WCDG hosts monthly public meetings from September to May. Meetings are currently virtual.
May 18, 2022 – Erik Regalado, Merck
Apr 18, 2022 – Glen Jackson, West Virginia University (Joint meeting with WBMSDG)
Mar 16, 2022 – Pawel Jaruga, NIST
Feb 15, 2022 – Dwight Stoll (Gustavus Adolphus College), Ben Niu (AstraZeneca)
Joint meeting with CASSS DC Discussion Group
Jan 19, 2022 – Leah G. Dodson, University of Maryland College Park
Ion Source Development for Radiative Association Kinetics Experiments
Recent advances in investigating radiative association (RA) reactions by quantum dynamics methods have revealed troubling discrepancies when compared with the reaction rates obtained using statistical methods, sometimes differing by up to four orders of magnitude. Notoriously difficult to measure in the laboratory, RA experiments are necessary to test the application of theoretical models to real systems. A new laboratory effort is being undertaken in the Chemistry Department at UMD to develop the tools necessary to experimentally measure rate constants for RA reactions at temperatures relevant to the interstellar medium (down to 10 K). The instrument combines a cryogenic buffer-gas beam with a cryogenic ion trap in order to investigate ion/molecule RA reactions as a function of temperature. In this talk, I will focus on the description of our glow discharge ion source and the general characteristics of our mass spectrometer. I will also describe our tactics for directly measuring the temperature-dependent reactivities with the immediate goal of measuring the rate constants for a series of RA reactions between magnesium monocations (Mg+) and cyanopolyyne (HC2n+1N, n = 0–3) neutral molecules. These experiments will shed light on disagreement between theoretical RA studies, while also providing experimentally-measured rate constants for reactions that are relevant to astrochemistry.
Bio: Leah grew up in central Pennsylvania. For undergrad she went to Case Western Reserve University in Cleveland, OH where she majored in Chemistry (minor in Physics) and graduated with a BS in 2010. While she was at Case, she studied the photophysical properties of pharmaceutical water pollutants in the research lab of Carlos Crespo-Hernández. Leah obtained her PhD in chemistry in 2016 with Mitchio Okumura at California Institute of Technology in Pasadena, CA. There she studied the spectroscopy and kinetics of radicals that are relevant to atmospheric and combustion chemistry, including several visits to the Advanced Light Source in Berkeley, CA and Sandia National Laboratories in Livermore, CA. After completing her degree, she moved to Boulder, CO where she was a NIST NRC postdoc in the lab of J. Mathias Weber at JILA/the University of Colorado. She carried out experiments using ion spectroscopy and cryogenic systems to obtain detailed structural information about gas phase ions. In Fall 2019, she started her independent group in the Chemistry Department at the University of Maryland in College Park, MD. Her group is focused on using cryogenic tools to study spectroscopy and kinetics of astrophysically-relevant molecules and ions.
Nov 17, 2021 – Robert D’Ippolito, NCI Frederick National Laboratory for Cancer Research
Refining the N-Termini of the SARS-CoV-2 Spike Protein and Its Discrete Receptor-Binding Domain
Previous work employing five SARS-CoV-2 spike protein receptor-binding domain (RBD) constructs, comprising versions originally developed by Mt. Sinai or the Ragon Institute and later optimized in-house, revealed potential heterogeneity which led to questions regarding variable seropositivity assay performance. Each construct was subjected to N-deglycosylation and subsequent intact mass analysis, revealing significant deviations from predicted theoretical mass for all five proteins. Complementary tandem MS/MS analysis revealed the presence of an additional pyroGlu residue on the N-termini of the two Mt. Sinai RBD constructs, as well as on the N-terminus of the full-length spike protein from which they were derived, thus explaining the observed mass shift and definitively establishing the spike protein N-terminal sequence. Moreover, the observed mass additions for the three Ragon Institute RBD constructs were identified as variable N-terminal cleavage points within the signal peptide sequence employed for recombinant expression. To resolve this issue and minimize heterogeneity for further seropositivity assay development, the best-performing RBD construct was further optimized to exhibit complete homogeneity, as determined by both intact mass and tandem MS/MS analysis. This new RBD construct has been validated for seropositivity assay performance, is available to the greater scientific community, and is recommended for use in future assay development.
Bio: Robert D’Ippolito is a Research Scientist supporting the NCI Ras Initiative at the Frederick National Laboratory for Cancer Research. His work primarily focuses on applying various proteomic techniques (e.g., top-down, crosslinking, and native mass spectrometry) to better understand the role of proteoforms and post-translational modifications in RAS-driven oncogenesis. Robert received his PhD in 2020 from Dr. Donald F. Hunt’s laboratory at the University of Virginia for developing a novel protein digestion method using a nonspecific enzyme reactor for middle-down proteomics.
Oct 20, 2021 – Elyssia Gallagher, Baylor University
Rapid H/D exchange-mass spectrometry for the analysis of glycans
Glycans are complex molecules with different carbohydrate subunits, linkage stereochemistries, and branching patterns; all of which play a role in their biological functions. Hydrogen / deuterium exchange–mass spectrometry (HDX-MS) has become a standard method for analyzing conformations and binding interactions of solvated proteins. Carbohydrates, model systems for glycans, are susceptible to HDX since they contain labile hydrogens, primarily in the form of hydroxyls, which can be labeled with deuterium (D) upon exposure to deuterated solvents. However, compared to backbone amides, the functional group detected in traditional HDX-MS experiments for proteins, the exchange rate of glycan hydroxyls is two to eight orders of magnitude faster, depending on solution pH. This rapid exchange rate makes it unfeasible to monitor HDX of carbohydrate hydroxyls using traditional, bottom-up HDX methods. Herein, we describe the development of rapid HDX methods to characterize carbohydrates. We perform rapid HDX by introducing deuterating reagents (e.g. D2O) to carbohydrates during electrospray ionization (ESI). Our work illustrates that these rapid, in-ESI HDX methods characterize solvated carbohydrates rather than gas-phase structures. Furthermore, we have coupled our experimental work with molecular dynamics simulations to identify the mechanism of carbohydrate ionization during ESI. Experimentally, we have quantified how ESI source conditions alter the magnitude of HDX for carbohydrate model systems, developed an internal standard to control for daily humidity differences, and established methods to alter the HDX labeling time on the microsecond to millisecond timescale. In this talk, we will discuss the validation of these methods and their preliminary applications, including the analysis of carbohydrate isomers.
Bio: Elyssia S. Gallagher is an Assistant Professor in the Department of Chemistry and Biochemistry at Baylor University (2015-present). Dr. Gallagher received her Ph.D. in Chemistry from the University of Arizona (2013) and was then awarded a National Research Council Postdoctoral Research Fellowship in the Biomolecular Measurement Division at the National Institute of Standards and Technology (2013-2015). The long-term goal of the Gallagher group is to advance the field of glycobiology through the development of novel analytical approaches that enable characterization of the structures of glycans and glycoconjugates to define their roles in diverse cellular processes. Current research focuses on the analysis of carbohydrates and protein-glycan binding interactions.
Sep 15, 2021 – Tongtong Xu, USP
Detection of undeclared sweeteners in steviol glycosides by LC/MS
Because of growing concerns about the need for added sugar reduction in food products and consumer’s preferences for “clean label” products, the food and beverage industry are taking their efforts towards calorie reduction through product reformulation using low- and no-calorie sweeteners from natural sources. Steviol glycosides, which are obtained from the leaves of Stevia rebaudiana Bertoni using various processing techniques, consist of a mixture of compounds containing a steviol backbone conjugated to sugar moieties. These products have been used globally as sweeteners in recent years, and the availability of various formulations created to suit the needs of a range of food manufacturers, as well as their consumer desirability have led to increased usage. In fact, the worldwide market for stevia extracts is expected to reach 700 million US dollars in 2024. The price of steviol glycosides is considerably higher than many other low- and no-calorie sweeteners, therefore making them vulnerable to adulteration with less expensive and less desirable high-intensity sweeteners. As a part of ongoing efforts to assist the food industry and regulators in combating economically motivated adulteration, the Food Chemicals Codex (FCC) has developed, validated and published a proposed LC-MS method to detect the presence of nine potentially undeclared low- and no-calorie sweeteners in steviol glycosides. As a source of global food standards, the FCC seeks to increase the role of public standards and guidance methods, including this publication, to protect consumers and food manufacturers from the growing trend of food adulteration.
Bio: Tongtong is a Senior Scientist supporting the Food Chemicals Codex (FCC) managing development and modernization of FCC standards for food ingredients, as well as the development of general tests detecting potential adulterants in vulnerable food ingredients. She formerly worked at the American Beverage Association monitoring the research landscape of the relevant ingredient safety and nutrition issues that may potentially impact the beverage industry. Before that, Tongtong worked in FDA evaluating the ingredients generally recognized as safe for their intended use in animal foods. She has a background in food science with more than 8 years of experience in the area of food ingredient safety, nutrition, and food additive regulations. Tongtong holds a Ph.D. in Food Science from the Pennsylvania State University.
May 19, 2021 – Roland F. Hirsch
How Analytical Chemistry, and especially Chromatography and Mass Spectrometry, is enabling progress in understanding climate
The Earth’s climate is a highly complex system. There is an urgent need for more accurate information about past climate and improved knowledge about the factors that influence climate today, in order to develop plausible models of future climate. Analytical chemistry, which plays a key role in studying past temperatures, rainfall, extent of ice and glaciers, and other relevant climate properties, is essential for gaining knowledge about climate-related processes that are occurring now in the atmosphere (such as formation and impact of aerosols). Mass spectrometry is the key technique for most of these studies. Chromatography, both gas and liquid, is also essential for many of these studies, usually in combination with mass spectrometry. This talk will discuss how these techniques are being used in climate research. Examples will be provided from the scientific literature.
Bio: Roland F. Hirsch lives in Overland Park, Kansas, with his wife of 49 years, Paula Jean. He received his Ph.D. in analytical chemistry from the University of Michigan. He joined the chemistry faculty of Seton Hall University, where he taught courses in general chemistry and analytical chemistry. In 1975-6 he was a Senior Visitor with Courtney Phillips at Oxford University, studying gas-solid chromatography. In 1984 he joined the Department of Energy as a program manager for analytical and separations chemistry. In 1988 he joined the civil service at NIH and in 1991 became a staff member at the DOE. He retired in 2018 and moved from Maryland to Kansas. He is a Fellow of the ACS, Honorary Fellow of the Library of America, and has been involved in ACS governance in the North Jersey Local Section and the Division of Analytical Chemistry.
Apr 19, 2021 – Stefani Thomas, University of Minnesota
Beyond the BRCA genes: A proteome-centric view of high-grade serous ovarian cancer
The mutational status of a solid tumor can predict the therapeutic efficacy of a specific drug in a molecularly defined subset of patients. Poly (ADP-ribose) polymerase inhibitors (PARPi) have emerged as a novel class of drugs to treat advanced ovarian cancer with mutations in BRCA1/2 genes. Unfortunately, there is considerable inter-patient heterogeneity in BRCA1/2–based determinations of PARPi treatment sensitivity. Determining proteome-level mechanisms of PARPi sensitivity could enhance our ability to select the ovarian cancer patient population that would benefit the most from PARPi therapy, consequently improving survival and overall treatment response. Our laboratory is applying mass spectrometry-based proteomics to identify protein signatures of PARPi sensitivity. This presentation will provide an overview of the experimental models and analytical approaches that we are utilizing toward a long-term goal of identifying prognostic protein biomarkers of PARPi sensitivity in patients with high-grade serous ovarian cancer.
Feb 17, 2021 – Nicholas Snow, Seton Hall University
GC in the 21st Century: Re-thinking Basic GC Ideas and Assumptions That We Grew Up With
Chromatography discussion groups have their origin in their name. They were formed in the 1950s and 1960s when chromatography was in its infancy and gas chromatography was dominant. Most of the theories, ideas and assumptions that we make in all chromatographic techniques have their origins in early GC work, as GC was the first instrumental chromatographic technique. The evolution of instrumentation for GC that finally takes full advantage of advances including capillary columns and selective detectors necessitates a look back at the fundamental assumptions and ideas that we all grew up with and that the book authors and short course instructors teach. This evening, we will discuss three fundamentals that we all think we know: carrier gas choice, gas hold-up time measurement and column polarity. Regarding carrier gas, we were all taught to use helium with capillary columns and if helium is not available, to use hydrogen. We suggest trying nitrogen, which we were taught not to use. The gas hold-up time is a critical measurement for nearly all calculations in GC. Back in the day, we were taught to inject methane or another supposedly non-retained substance to measure this value. With today’s automated systems, manually injecting methane is not a simple matter. We will look at alternatives to injecting methane and discuss possible impacts of variability in this measurement on further calculations. Finally, “polarity” is a broad term that is often conflated with retentiveness; a more polar column is often considered more strongly retentive. We will examine column polarity more closely and see how the classical definition and usage is often confusing.
Bio: Nicholas H. Snow is Founding Endowed Professor of Chemistry and Biochemistry at Seton Hall University and Adjunct Professor of Medical Science at Hackensack UMC Medical School in New Jersey. He is a proud alumnus of Kent Gardens Elementary School, Longfellow Middle School and McLean High School in McLean, Virginia, so this is a homecoming of sorts. Prior to moving to New Jersey in 1994, he continued his education on Virginia with a BS in Chemistry from UVA, a PhD at Virginia Tech and postdoctoral work and lecturing back at UVA. For over 25 years he has led an active research group with 80 publications and two books, mostly related to GC. He is the GC Connections Editor for LCGC Magazine and Associate Editor of the Journal of Liquid Chromatography and Related Technologies. He is co-author of the latest edition of McNair’s classic book “Basic Gas Chromatography”. He holds Seton Hall’s McQuaid Medal for exemplary service by a long-term employee and he was recently recognized for 25 years of service on the Governing Board of the Eastern Analytical Symposium.
Jan 20, 2021 – Camille Lombard-Banek, NIST-IBBR
Building a DIA-based Proteomic Workflow for the Characterization of CAR T-cell Therapies
CART T-cell therapies are novel cancer treatments, whereby the patient’s T-cells are engineered to express chimeric antigen receptors (CARs) on their surface. Three CAR T-cell therapies have recently received approval by the Food and Drug Administration for the treatment of refractory lymphomas, albeit as last resort solutions. To promote the broader adoption of this new therapeutic modality, innovative technologies are needed, especially ones based on mass spectrometry (MS)-based proteomic. Here, we present the development of a sensitive proteomic method using data-independent acquisition, to help better define critical quality attributes (CQAs) of the cells and final product attributes (PQAs). We evaluated multiple key steps of the data acquisition from separation to detection to enhance the sensitivity by data dependent and independent acquisition. Moreover, we developed a cell model to revise the protein extraction workflow. We also present suggestions for best practices in instrument qualifications (IQ), to facilitate the adoption of large-scale proteomics to the biopharma space. The data represent a benchmark that will be used as IQ metrics to assure instrument control for biologically relevant decision making on CAR-T proteomic samples.
Bio: Camille Lombard-Banek is a NRC postdoctoral fellow at NIST/IBBR in the laboratory of John Schiel where she is developing liquid chromatography mass spectrometry-based proteomic approaches for the analysis of emerging cell and gene therapies. She obtained her PhD from the George Washington University developing micro-analytical tools for the analysis of single embryonic cells in Peter Nemes’ laboratory.
Dec 16, 2020 – Kim Haynes, Waters Corporation
ACQUITY PREMIER: Precision Chemistries for Particles and Surfaces
LC-based analysis of metal sensitive analytes, such as those containing phosphate groups and deprotonated carboxylic acids, can be challenging due to their interactions with metal components within a chromatographic separation. These interactions can lead to missed analytes, quantification errors, and wasted time. In this seminar, you will learn how ACQUITY PREMIER Columns with MaxPeak High Performance Surfaces enables scientists to see compounds previously unseen, while improving sensitivity and reproducibility for all analytes. In addition, it will be shown how this technology can reduce the need for lengthy and repeated passivation.
Bio: Kim Haynes is currently a Principal Product Marketing Manager in the Chemistry Technology Center at Waters Corporation. Kim manages MaxPeak High Performance Surface Technologies as well as a variety of other programs. Prior to this position, she has worked with Waters Corporation as a chemistry sales specialist and a business development manager. During her 20 years at Waters, Kim has focused on helping customers and colleagues understand chromatographic separations and sample preparation. Before joining Waters Corporation, Kim worked as an analytical chemist at Bacardi Martini. She holds a BS in Chemistry from Ohio University. She can be contacted at email@example.com.
Nov 18, 2020 – Geoffrey Dubrow, FDA Center for Food Safety and Applied Nutrition
A Survey of Cannabinoids in Hemp-Derived Products from the United States Marketplace
The 2018 Agricultural Improvement Act removed hemp from Schedule I control, creating a market for hemp products, including cannabidiol-containing products. Due to the market’s rapid growth, little is known about the presence and concentration of cannabinoids in commercial products. In the present study, 11 cannabinoids were quantified using liquid chromatography with diode-array detection in a non-representative sampling of 147 products labeled as containing hemp or cannabidiol. Cannabinoid content ranged from 0–143 mg/serving, with a median of 16.7 mg/serving. Fewer than half of products surveyed contained cannabidiol concentrations within 20% of their label declarations. Most products presented concentrations of ∆9-tetrahydrocannabinol below LOQ. These findings emphasize the need for further testing and representative investigation of the cannabidiol marketplace.
Bio: Dr. Geoffrey A. Dubrow is a staff fellow in the Office of Regulatory Science within FDA’s Center for Food Safety and Applied Nutrition. At CFSAN, his work focuses on the analysis of cannabinoids in hemp-containing products. Prior to his work at FDA, Geoff completed his PhD in food science and technology at the Ohio State University in 2019, focusing on the use of untargeted LC/MS and GC/MS “flavoromics” to understand chemical drivers of consumer acceptability in strawberry jams. Beyond food safety, his research interests include the application of chemometrics and metabolomics towards the understanding of food and flavor chemistry, and high-throughput sample preparation.
Oct 21, 2020 – Nadja B. Cech, University of North Carolina at Greensboro
Challenges and Opportunities in Transitioning from Targeted to Untargeted Analysis in Small Molecule Mass Spectrometry
Mass spectrometrists and chromatographers have spent more than 100 years developing better tools and methods to separate and identify the compounds in mixtures. Historically, much of our field was focused on identifying and quantifying specific known constituents in those mixtures. More recently, there has been a shift in interest towards “untargeted” or “nontargeted” techniques, in which the analyst seeks to capture a more comprehensive profile of the molecules present in a mixture. This talk will address some of the challenges and opportunities our fields have encountered in transitioning from targeted to untargeted approaches, particularly focusing on small molecules (metabolomics). Specific case studies will be included from ongoing research in the Cech Laboratory, where we seek to employ untargeted metabolomics to identify antimicrobial constituents in complex extracts from plants and fungi.
Bio: Dr. Nadja B. Cech is Patricia A. Sullivan Distinguished Professor of Chemistry at UNC Greensboro. She applies her expertise in mass spectrometry to solve challenging problems in natural products research, largely supported with funding from the National Institutes of Health. Work in the Cech group focuses on the application of mass spectrometry metabolomics strategies to identify biologically active constituents from complex mixtures and to understand virulence in bacterial pathogens. Dr. Cech supervises a research group of eighteen students and postdoctoral research associates and is currently schooling and feeding two children during the pandemic. She is the recipient of the 2011 Jack L. Beal Award for Best Paper in the Journal of Natural Products by a Young Investigator, and the 2017 Thomas Norwood Award for Undergraduate Research Mentorship. Dr. Cech is a member of the research team for the NCCIH-funded Center of Excellence for Natural Product Drug Interaction, and Co-Director of the Medicinal Chemistry Collaborative.
Sep 16, 2020 – Asif Shajahan, University of Georgia
How advanced mass spectrometry can guide the therapeutic interventions against SARS-CoV-2
Apr 20, 2020 – Ben Neely, NIST
Sea lions and bats and humans, oh my! How to explore mammalian serum proteomes
Feb 19, 2020
Jie Li, University of Maryland College Park
LC-HRMS Based Metabolomic Analysis of Neural-Tissue Fated Cell Clones from Developing Xenopus laevis Embryos
Alexander Zestos, American University
LC-MS/MS Method for Neurochemical Detection in Biological Samples
Jan 15, 2020 – Ed Sisco, NIST
Utilizing Trace Drug Residues in the Forensic Analysis of Opioids
Dec 18, 2019 – Hugh Hayes, NIST
Method development for the quantitation of key analytes in environmental extracts and botanical dietary supplementation reference materials
Nov 14, 2019 – Lane C. Sander, NIST
Stationary Phase Architecture and Molecular Shape Differentiation in Liquid Chromatography
Oct 16, 2019 – Paulina Piotrowski, NIST
Characterizing the Gut Microbiome
Sep 18, 2019 – Annual Poster Session & Vendor Exhibition
Bethesda North Marriott Conference Center, 5701 Marinelli Rd, Rockville, MD
Congratulations to Jie Li from University of Maryland for winning the Guiochon Student Award for his poster titled “Metabolic Characterization of Cell Clones in X. laevis Embryos by HPLC-MS”.
May 15, 2019 – Horacio Pappa (USP) and Fadi Alkhateeb (Waters)
Method Lifecycle Management
Apr 15, 2019 – Catherine Fenselau, University of Maryland
Biological Mass Spectrometry: Observations on its Evolution
Mar 13, 2019 – William Craig Byrdwell, Beltsville Human Nutrition Research Center, USDA
Comprehensive Multi-Dimensional Chromatography with Quadruple Parallel Mass Spectrometry for Fat-Soluble Vitamins and Triacylglycerols
Feb 27, 2019 – Erika Portero, University of Maryland College Park
Capillary Electrophoresis Mass Spectrometry for Microanalysis of Proteins and Metabolites in the Developing Vertebrate Embryo
Jan 16, 2019 – James P. Grinias, Rowan University
Increasing Throughput for Pharmaceutical and Bioanalytical Methods Using UHPLC
Dec 12, 2018 – Perry Wang, FDA
Identification and Quantitation of 16 PAHs in Tattoo Inks by UHPLC-APPI-MS/MS with Scheduled Selected Reaction Monitoring
Nov 14, 2018 – Andre Striegel, NIST
Multi-detector Hydrodynamic Chromatography of Colloids and Polymers
Lucas Kimerer, University of Virginia
Oct 11, 2018 – Melissa Phillips, NIST
Application of Chromatography in the Development of Reference Materials for Foods and Dietary Supplements
Sep 19, 2018 – Annual Poster Session & Vendor Exhibition
Bethesda North Marriott Conference Center
Congratulations to our student poster winner Lucas Kimerer (University of Virginia). He will receive a travel award to attend HPLC 2019 in Milan, Italy, and he will give short oral presentation at a future WCDG meeting.
May 29 to August 2, 2018 at Marriott Wardman Park, Washington, DC
WCDG sponsored the musical entertainment for the opening ceremony and reception. During the opening ceremony, The Conservatory Classic Jazz Band delighted conference attendees with jazz numbers inspired by various regions around the world to celebrate the international community of separation scientists gathered for HPLC 2018. During the evening reception, attendees were serenaded with a vocalist and guitarist. WCDG also congratulates Associate Professor Peter Nemes of UMD for receiving the Georges Guiochon Faculty Award.
May 16, 2018 – Ira Lurie, George Washington University
Recent Developments in the Analysis of Emerging Drugs
Apr 16, 2018 – Melanie Downs, University of Nebraska-Lincoln
MS Analysis of Food Allergens: Just Another Proteomics Application or a Whole New Kettle of Fis
Mar 14, 2018 – Mark R. Schure, Kroungold Analytical, Inc., Blue Bell, PA
Multidimensional separations in three and higher dimensions
Sarah Gao, 2017 Student Poster Winner
Feb 15, 2018 – Chunlei Wang, MedImmune
Efficient peak identification for hydrophobic interaction chromatographic separations by 2DLC/MS
Hangu Nam, 2017 Student Poster Winner
Jan 17, 2018 – David Muddiman, North Carolina State University
Innovations in Mass Spectrometry Platform Technologies for Epithelial Ovarian Cancer Research and High Throughput Screening
Dec 6, 2017 – Joseph Zaia, Boston University
How to assign protein site-specific glycosylation
Nov 15, 2017 – Gary Mallard, NIST
Automating GC/MS Analysis – Or at least making it a little easier
Oct 18, 2017 – John Hanover, NIDDK, NIH, Bethesda, MD
A Little Sugar goes a Long Way: O-GlcNAc in human disease
Sep 20, 2017 – Annual Poster Session & Vendor Exhibition
Bethesda North Marriott Hotel & Conference Center
Congratulations to our student poster winners Sarah Gao (IBBR) and Hangu Nam (Virginia Tech). They will receive travel awards to attend HPLC 2018, and they will give short oral presentations at a future WCDG meeting.
Aug 22-23, 2017 – Short Course: Capillary Electrophoresis Workshop
Instructor: Brandon Bates, Sciex
May 17, 2017 – Christian Wolf, Dept. of Chemistry, Georgetown University, Washington, DC
Chromatographic and Spectroscopic Analysis of Chiral Compounds
Apr 19, 2017 – Walter B Wilson, Research Chemist, NIST
Liquid and gas chromatographic retention behavior of polycyclic aromatic compounds on stationary phases of different selectivities
Rosemary Onjiko & Erika Portero, 2016 Student Poster Honorable Mentions
Mar 27, 2017 – Robert N. Cole, Assistant Professor Dept. Biological Chemistry, Johns Hopkins School of Medicine
Adventures of Capillary Electrophoresis in Proteomics from Top-down to Bottom-up
Feb 15, 2017 – Peter Nemes, Assistant Professor, Dept. of Chemistry, George Washington University
Capillary Electrophoresis Mass Spectrometry for Understanding Early Vertebrate Embryonic Development One Cell at a Time
Sam Choi, 2016 Georges Guiochon Poster & Travel Award recepient
Jan 18, 2017 – Abigail Turner, National Institute of Standards and Technology
The NISTmAb: Capillary Electrophoresis-based Characterization and Life Cycle Management
Nov 17, 2016 – Mark L. Miller, FBI Counterterrorism and Forensic Science Research Unit
Challenges in Forensic Separations
Oct 19, 2016 – Trina Formolo, National Institute of Standards and Technology
Considerations for Optimization of Multi-Attribute Peptide Mapping Protocols
Sep 21, 2016 – Open House & Poster Session
North Bethesda Marriott Hotel and Conference Center
Congratulations to Sam Choi, our 2016 winner of the Georges Guiochon travel award for best student poster!
Sam Choi from George Washington University won the award for his outstanding poster on “CE-nanoESI-MS for Untargeted Proteomic Characterization of Single Embryonic Cells and Small Neuron Populations”. He will receive complementary travel to a separations-related conference of his choice. Congratulations, Sam!.
Honorable Mentions were Erika Portero and Rosemary Onjiko from George Washington University.
May 18, 2016 – Milton L. Lee, Brigham Young University
Compact Capillary LC Separations
Apr 11, 2016 – Stephen E. Stein, Director of NIST Mass Spectrometry Data Center
Mass Spectral Libraries of Everything
Feb 17, 2016 – Robert diTargiani, U.S. Army Medical Research Institute of Chemical Defense
Analytical Methods for the Verification of Human Exposure to the Chemical Warfare Agents
Jan 20, 2016 – Steve J. Lehotay, USDA-ARS Eastern Regional Research Center (ERRC), Wyndmoor, PA
Improving the quality of chromatographic peak integration and analytical results without human review in multi-analyte high-throughput methods
Dec 16, 2015 – Ronald E. Majors, LCGC North America, West Chester, PA
Top Ten HPLC/UPLC Column Myths
Nov 18, 2015 – Claude R Mallet, Waters Corporation
Optimized Workflow for Metabolite Identification using 3D LC/MS/MS
Jason Reck, University of Virginia
Oct 21, 2015 – Irving W. Wainer, Mitchell Woods Pharmaceuticals
The adventures of a bioanalytical chemist in the never-never land of antidepressants: Finding a way out of the “ketamine paradigm”
Bethesda North Marriott Hotel
Camille Lombard-Banek (George Washington Univeristy) received Honorable Mention and a $500 award for travel. Contact: firstname.lastname@example.org
May 20, 2015 – Jianghao Sun, Food Composition and Method Development Lab Beltsville Human Nutrition Center, USDA-ARS
Fingerprinting and Metabolomics for Botanical Authentication and Identification of Dietary Biomarkers
Apr 15, 2015 – Matthew C. Crowe, The Dow Chemical Company, Philadelphia, PA
Multidimensional Liquid Separations Coupled to Electrospray Ionization Mass Spectrometry for the Analysis of Complex Polymers
Mar 10, 2015 – Chromatography Community Mixer at PittCon
Generations Hall, 310 Andrew Higgins Dr, New Orleans, LA
Feb 11, 2015 – Rabih Jabbour, Edgewood Chemical Biological Center
Utilization of a Mass Spectrometry Proteomics Method for Detection of Biological Agents and Toxins in Complex Matrices
Jan 21, 2015 – Jeffrey W. Hudgens, NIST
Hydrogen-Deuterium Exchange Mass Spectrometry: A Valuable Tool for the Biopharmaceutical Industry
Dec 17, 2014 – Bill LaCourse, University of Maryland Baltimore County
Adventures in Analysis: New Approaches to Old Problems
Nov 12, 2014 – Abul Hussam, George Mason University
Measurement of Noncovalent Association and Partitioning of Volatile Solutes by Equilibrium Headspace Gas Chromatography: Applications in Solvent Extraction, Surfactants, and Nanoparticles
One of our most prestigious members has passed away. Georges Guiochon, a previous WCDG President, after defeating pneumonia finally succumbed to neuromuscular failure due to Post Polio Syndrome on October 21, 2014. He was 83 years old and still working at UT Knoxville until the day before he entered intensive care on October 7.
Arrangements are underway for Memorials to be held in Paris, Buffalo, and UT Knoxville.
With warmest regards from another previous WCDG President, Lois Ann Beaver, surviving spouse of Georges Guiochon.
A remembrance from Journal of Chromatography A: www.journals.elsevier.com/journal-of-chromatography-a/news/professor-georges-guiochon-1931-2014-passed-away-on-october/
Oct 15, 2014 – Peter Nemes, George Washington University
Metabolic Analysis of Single Cells in Embryos by Mass Spectrometry
May 21, 2014 – Theodore Juarez, Uniformed Services University
Inter-variability of INFICON HAPSITE ER Instruments (man-portable Gas Chromatograph/Mass Spectrometer) operated with Manufacturer Provided Calibration Curves
Apr 14, 2014 – Maureen Kane, University of Maryland, School of Pharmacy
The use of fast HPLC multiplexing MRM3 for retinoic acid quantitation in complex matrices
Tuesday, March 4, 2014
5:30 – 8:30 PM
Buddy Guy’s Legends, 700 S Wabash Ave, Chicago, IL
Resolution of Sialylated Glycopeptides Using a Pentafluorophenylpropyl (PFP) Stationary Phase
Feb 12, 2014 – Fabrice Gritti, University of Tennessee
Importance and constraints of the recent progress made in column and instrument technologies for accurate sample quantification by HPLC
Dec 11, 2013 – Isiah Warner, Ph.D., Louisiana State University, Baton Rouge, LA
GUMBOS: Tunable Materials for Biomedical Applications
Nov 13, 2013 – Tony Brand, Agilent Technologies
Do we really know what the left and right are up to? Supercritical Fluid Chromatography is now the tool to tell, with some help from an MS
Oct 23, 2013 – Benjamin Place, NIST
Multi-Dimensional Chromatography as a Quantitative Tool
Bethesda North Marriott Hotel & Conference Center
May 22, 2013 – Christopher Okunji, USP
High Speed Counter-Current Chromatography: An Efficient Technique for Large-Scale Isolation of Pharmacopeia Reference Standards
Apr 15, 2013 – Michael Boyne, FDA
Modern Analytics for the Analysis of Complex Drug Products: Physicochemical Characterization using MS
Feb 20, 2013 – Andrew Alpert, PolyLC
Advantages of HILIC and ERLIC for Proteomics
Jan 16, 2013 – Kevin Siek, LECO Corporation
Sharpening Chromatographic and Spectral Resolution to Reveal More in Metabolomic, Food Safety, Environmental, and Pharmaceutical Sciences
Dec 12, 2012 – Jeff Rohrer, Thermo Fisher Scientific
Ion Chromatography for Pharmaceuticals
Nov 13, 2012 – Len Sidisky, Supelco
Advances in Ionic Liquid Stationary Phases for Capillary Gas Chromatography
Oct 11, 2012 – Jessica Reiner, NIST
Temporal and Spatial Trends of Current-use and Legacy Persistent Organic Pollutants in Marine Mammals
Sep 12, 2012 – Open House & Poster Session
May 16, 2012 – Pierluigi DelMonte, Office of Regulatory Science Center for Food Safety and Applied Nutrition, FDA
Investigation of comprehensive LCxLC for the separation of phytochemicals in dietary supplements
Apr 18, 2012 – Mark Lowenthal, NIST
Developing LC-MS methods for qualitative characterization of NIST Standard Reference Materials
Mar 21, 2012 – John Van Antwerp, Waters Corporation
Fundamentals and Advancements in Analytical SFC
Feb 15, 2012 – Rick Lake and Ty Kahler, Restek Corporation
Characterizing Stationary Phase Selectivity – A Practical Look at Column Selection
Jan 18, 2012 – Ruin Moaddel, National Institute on Aging, NIH
Bioaffinity Chromatography: The Study of Drug-Protein and Protein-Protein Interactions
Nov 16, 2011 – Cynthia Tyburczy, Ph.D., FDA-CFSAN
Covalent Adduct Chemical Ionization Mass Spectrometry for the Identification of Fatty Acid Methyl Ester Double Bond Configuration
Apr 20, 2011 – Manuela Gradl, NIST – Walking on Ceilings: Chemistry of Insect Adhesion to Various Surfaces
Jacolin Murray, NIST – Field Sampling and Detection Using a Novel Portable Gas Chromatograph-Mass Spectrometer
Mar 21, 2011 – Kevin Schug, University of Texas Arlington
Five Years, Three Boys, and Some Analytical Chemistry
Feb 16, 2011 – Lane Sander, NIST
Order and Disorder in Chromatography