Washington Chromatography Discussion Group (WCDG) meets monthly from September to May. Meetings are currently being held virtually.
January 20, 2021
5:00-5:30PM Breakout Sessions
Speaker: Camille Lombard-Banek, NIST & IBBR
Title: Building a DIA-based Proteomic Workflow for the Characterization of CAR T-cell Therapies
Abstract: CART T-cell therapies are novel cancer treatments, whereby the patient’s T-cells are engineered to express chimeric antigen receptors (CARs) on their surface. Three CAR T-cell therapies have recently received approval by the Food and Drug Administration for the treatment of refractory lymphomas, albeit as last resort solutions. To promote the broader adoption of this new therapeutic modality, innovative technologies are needed, especially ones based on mass spectrometry (MS)-based proteomic. Here, we present the development of a sensitive proteomic method using data-independent acquisition, to help better define critical quality attributes (CQAs) of the cells and final product attributes (PQAs). We evaluated multiple key steps of the data acquisition from separation to detection to enhance the sensitivity by data dependent and independent acquisition. Moreover, we developed a cell model to revise the protein extraction workflow. We also present suggestions for best practices in instrument qualifications (IQ), to facilitate the adoption of large-scale proteomics to the biopharma space. The data represent a benchmark that will be used as IQ metrics to assure instrument control for biologically relevant decision making on CAR-T proteomic samples.
Bio: Camille Lombard-Banek is a NRC postdoctoral fellow at NIST-IBBR in the laboratory of John Schiel where she is developing liquid chromatography mass spectrometry-based proteomic approaches for the analysis of emerging cell and gene therapies. She obtained her PhD from the George Washington University developing micro-analytical tools for the analysis of single embryonic cells in Peter Nemes’ laboratory.
2021 Virtual Student Symposium & Vendor Exhibition
March 17, 2021
Washington Chromatography Discussion Group (WCDG) invites you to our Virtual Student Symposium & Vendor Exhibition. This event is FREE to attendees.
Student with best oral presentation will receive the Guiochon Student Award, stipend to attend a separations conference, and the opportunity to present at a WCDG meeting.
Corporate sponsors of WCDG will be given the opportunity to show promotional videos between oral presentations. Email email@example.com for more information about sponsorship or the event.
New to analytical chemistry or looking to sharpen your skills? Visit NIST’s site for videos and PowerPoints on Tutorials in Analytical Chemistry